Sclerotioramine is an azaphilone pigment prepared synthetically from sclerotiorin and reported by Whalley and co-workers at the University of Liverpool in 1957. Subsequently, sclerotioramine was isolated as a natural product from various Penicillium species. Sclerotioramine is active against Gram positive bacteria, producing modest zones of inhibition against Staphylococcus epidermidis in a disk diffusion assay at a concentration of 30 μg/6 mm paper disk. Sclerotioramine inhibited Candida albicans, C. parapsilosis, C. tropicalis, and C. krusei (8, 7, 8, and 8 mm zones of inhibition, respectively). Sclerotioramine has moderate activity (IC50 16.31 μg/ml) in vitro against HepG2 cell line and has comparable activity to acarbose against α-glucosidase.