Sporidesmolide II is the most abundant of N-methyl analogues belonging to the hexadepsipeptide sporidesmolide complex. Biosynthesis of sporidesmolide II involves the use of D- and L- amino acids utilising D-alloisoleucine, leucine and four valine sub-units, where two of the valines are converted to the corresponding valic acid. The biological activity of sporidesmolide II has not been extensively investigated.