Penicolinate B is a picolinic acid derivative isolated from Penicillium sp.. Penicolinate B is potently against Plasmodium falciparum and Candida albicans (IC50 1.45 µg/mL in each assay) and has moderate activity against Mycobacterium tuberculosis and Bacillus cereus (25 µg/mL in each assay). Penicolinate B is also active against the tumor cell lines KB (human epidermoid carcinoma, IC50 18 µg/mL), MCF-7 (human breast cancer, IC50 6 µg/mL), NCI-H187 (human small cell lung cancer, IC50 0.3 µg/mL) and Vero (IC50 2.8 µg/mL).