The nanangenines are newly discovered drimane sesquiterpenoid metabolites from a hitherto undescribed Australian fungus, Aspergillus nanangensis. Nanangenine D is a drimane lactone bearing a C6 acyl side chain. Nanangenine D has potent activity against B. subtilis (IC50 5.7 μg/mL) and NS-1 cell line (IC50 4.1 μg/mL). but was inactive against tumor cell lines (mouse myeloma NS-1 cell, human prostate DU-145 and human breast adenocarcinoma MC-7) and neonatal foreskin. The nanangenines did not exhibit Gram negative or antifungal activity.