The aminoglycosides are an important family of potent Gram-negative and Gram-positive antibiotics produced by Streptomyces and Micronospora sp. Aminoglycosides act through inhibition of protein synthesis and have been a mainstay for treatment of serious bacterial infections for 80 years. Their core structure consists of amino sugars connected via glycosidic linkages to a dibasic aminocyclitol. The Aminoglycoside Metabolite Set includes the first-in-class streptomycin and examples of the four sub-classes which are based on the substitution of aminocyclitol moiety. The set also includes the chemically-related aminocyclitol, spectinomycin.