Research on the avermectin class of nematocides has spanned 35 years with thousands of analogues reported in the patent and scientific literature. The six analogues in the Avermectin Analogue Set represent pivotal commercial advances in the development of this class, with the analogues displaying a range of physico-chemical properties, potencies and spectrum of action as anthelmintics and acaricides. The Avermectin Analogue Set is a useful tool in addressing current research questions on mode of action, species selectivity and cross resistance within this exciting class of macrocyclic lactones.