N-Acetylserotonin is an acetyl derivative of a biogenic acid commonly found in many microbial fermentations, most notably dermatophytic fungi belonging to the genus, Trichophyton. N-Acetylserotonin is produced by decarboxylation and acetylation of serotonin. N-Acetylserotonin exhibits weak activity in a diverse range of bioassays and is thus an important standard for analytical and bioassay dereplication of crude microbial extracts.