3'-Ethoxyspinosyn L 17-pseudoaglycone is an acid degradation product produced by selective hydrolysis of the more labile forosamine saccharide in the 17-position of 3'-ethoxy-5,6-dihydrospinosyn J, the minor component of the commercial product, Spinetoram. 3'-Ethoxyspinosyn L 17-pseudoaglycone is only weakly active as an insecticide as the forosamine moiety is considered essential for potent activity. Despite the importance of spinosyns as agro-chemical insecticides, there are few published reports of the biological activity or the levels of 3'-ethoxyspinosyn L 17-pseudoaglycone in the environment.